MELTWIN 3.5 SCREEN SHOTS

FEATURES & SPECIFICATIONS

• MAIN FEATURES
  • Extraction of thermodynamic parameters from nucleic acid melting experiments using the following analyses:
    • Standard 2-state (duplex/single strand) model
    • Ising models for single-stranded and non-self complementary equilibria
  • Tm versus concentration dependence analysis for bi-molecular equilibria
  • Curve shape analysis eliminates entry of initial parameters sets greatly simplifying the fitting process
  • Built-in extinction coefficient calculations for easy strand concentration calculation
    
  • Wizard for automated fitting: Encapsulates fitting procedures automating most of the process making data analysis fast and easy with almost no knowledge of the theory required

• GRAPHICS
  • Absorbance vs. Temperature plots for single cells
  • Normalized Absorbance vs. Temperature plots for multiple cells
  • 1/Tm vs. log(Ct) plots for bi-molecular systems
  • Graphical data editing for removing bad point or regions from fittings
  • Complete curve formatting including customizing colors, fonts, point types & weights, and zooming & sizing of plots.

• ANALYSES
  • Non-linear analysis of 2-State and Ising models
  • Proprietary curve shape analysis for easy non-linear fitting of most data
  • DISPO (digital smoothing polynomial) data filtering for accurate fitting of noisy data
  • Nearest-neighbor extinction coefficient calculation for concentration determination
  • Linear regression analysis of Tm/Concentration dependence

• ADDITIONAL
  • Projects files for grouping multiple data files
  • Comma-separated value (CSV) data filter for easy importing of data from various sources and spectrometer acquisition programs
  • Add Data Points feature for introducing additional fitted data from different data files (useful for large concentration studies)
  • Printing support
  • Full help including descriptions and references of analyses

• SYSTEM REQUIREMENTS
  • PC with a 486DX/66 MHz or higher processor (Pentium or higher recommended)
  • Microsoft Windows 95, Windows 98, Windows ME, Windows NT Workstation 4.0 (with Service Pack 2), Windows 2000, Windows XP
  • 16 MB of RAM
  • 3 MB of hard disk space
  • VGA or higher-resolution monitor
  • Microsoft Mouse compatible pointing device